I'm interested in using a Barley Affy array annotation, obtained with Blast2GO and converted to the GSEA program, for enrichment analysis and to represent the data with Cytoscape? I am also interested in alternatives.
Not sure about how to convert the output from Blast2GO, however if you are using public or private Affy gene expression arrays you can use InSilico DB (https://insilicodb.org) to seamlessly export your datasets to the genepattern GSEA module, and to GenomeSpace which is integrated with Cytoscape.