We are planning to conduct a scoping review, but we are not sure if review of related literature is needed or not. We've read articles and we only saw Introduction and then Methods. If RRL is needed, what should be the general content of it?
Hopefully can doing so.
Hello all, I'm trying to use electrophoresis to separate 3 operons that are 500-600bp, for gel extraction and direct sequencing. When I check my PCR products on a 1% agarose TBE gel, I have clear...
04 December 2022 8,209 7 View
Hello everyone, I amplified from cDNA template my sequence of interest using SuperFi II polymerase and I obtain two bands, one at 1300bp (the weight that i want to have) and other strong band at...
21 July 2021 9,235 12 View
Hi! I need the price per cm2 of cation exchange membrane of polyethylene styrene-divinylbenzene used in microbial fuel cell (MFC) systems. Or if you know a page where this is for sale it would...
01 September 2020 4,317 0 View
Hi researchers, I've been working with my own mucin obtained from porcine's gut and I'm trying to purify it by my own. The thing is that when I do dilutions of the fecal sample, and inoculate...
17 December 2019 1,808 0 View
I would like to measure enzymatic activity of some nuclear proteins, but I have troubles with the extraction and keeping its native form. Sonication seems to be a must in my cells to release...
23 September 2019 7,615 7 View
Hi, I want to quantify DNA, I have a GENESYS 10S UV-Visible and Visible spectrophotometer, but I do not have the instructions. Could someone tell me what to do? Do I need some software? Thank you
10 August 2018 6,380 2 View
I need a working protocol to extract DNA from Salmonella colonies.
07 June 2018 7,525 3 View
Hi, I am new to primary cell culture and I'll be working with chicken-derived dendritic cells. I'll be using GM-CSF and IL-4 to induce their maturation in 6-well plates with RPMI-1640 media. I...
05 February 2018 4,053 4 View
Hi, I'm working on a project where the main objective is to measure the maturation markers of chicken-derived dendritic cells. Naturally, I am looking at CD40, CD80/86 and MHCII markers via flow...
05 February 2018 2,581 3 View
NASA's Mars rover Curiosity captured this image on Jan. 2, 2018, with its Mars Hand Lens Imager (MAHLI). Using an onboard focusing process, the robot created this product by merging two to eight...
10 January 2018 4,823 10 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
What is the future scope of acoustic emission?
17 July 2024 1,510 1 View
We invite researchers, academics, and practitioners from various fields to join us in a collaborative effort to write multidisciplinary papers. The goal of this initiative is to combine...
01 July 2024 6,083 7 View
cloud forensics
23 June 2024 9,811 1 View
I am deciding on search terms for a scoping review. I am planning searching 3 multidisciplinary databases and currently getting about 5000 hits from my search terms in the first database. What do...
19 June 2024 7,417 0 View
Asper discussion, First time my clonning was failed becuase the first 45 nucleotide was not cloned in pet30 a vector then I choosed the different clone of 1:1 ration fraction, I got the size of...
13 June 2024 7,564 7 View
I am working with chitosan/dextran sulphate nanoparticles loaded with rifampicin, which is fluorescent. Dsl analysis are bad and I saw that is necessary use a narrow band filter. How can I select...
05 June 2024 1,324 3 View
All the topics should be mostly new in the last 1-2 years and have industrial and academic value and scope. For example: Responsible AI. Please don't give chatgpt or other AI-based ans.
30 May 2024 7,503 2 View