How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

HiPSC positive for GLAST in FACS - why?

Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...

09 February 2021 3,383 4 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Are these IPScs Differentiated ?

Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...

28 December 2020 3,503 3 View

How to stain iPSC derive motor neuron in 96-well plate without detachment?

Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...

14 July 2020 3,828 7 View

Why does my H9 IPSC die reasonless after 80% confulency?

Dear All, I am working with thermo fisher H9 cell line, human induced pluripotent stem cell. I made a mutation on them with crispr cas-9 I obtain single colonies and I propagate my clones. I...

13 June 2020 9,046 3 View

Is this be a fungal/bacteria contamination in iPS cell culture?

Dear colleagues, I'm working with iPS cell culture for the first time and after dissociating and placing them in a plate coated with matrigel, they looked like the photo. Could anyone tell me if...

21 December 2019 2,888 14 View

Is this be a fungal/bacteria contamination in iPS cell culture?

Dear colleagues, I'm working with iPS cell culture for the first time and after dissociating and placing them in a plate coated with matrigel, they looked like the photo. Could anyone tell me if...

11 December 2019 3,570 6 View

How to eliminate a single component from a medium?

I'm planning to change the components of cell culture media for reprogramming iPSC in order to make it cost-effective for lab research. some research was made in related areas, but my question is...

10 November 2019 9,457 3 View

Why there is no neutrophil progenitors in human as Hoxb8cells in mice which can produce neutrophils?

As question above is there any certain reason that makes human neutrophil progenitors which can produce neutrophils unavailable? I only know HL60 cells and people differentiate neutrophils from...

10 November 2019 7,763 1 View

How can i know -preferably by morphology- that EB are mature and ready for the next step of differentiation?

I am working on differentiating hiPSC into macrophage through EB method, and i wonder how the EB should look like and what are the characteristics before it is cultured with monocytes...

10 November 2019 3,465 2 View