Does retention time really become longer with increasing alcohol content in chiral separation by HPLC?

21 February 2015 1 8K Report

If there is a competition of the alcohol and analytes for H-bonding we can expect reduction of retention

DEAR NASSER

USUALLY INCREASING THE ALCOHOL CONTENT IN THE MOBILE PHASE CAUSE A DECREASE IN THE RETENTION TIME. HOWEVER IF THE MOBILE PHASE CONTAIN A MORE POLAR SOLVENT THAN ALCOHOL e.g. WATER AS IN THE CASE OF REVERSED PHASE MODE THEN THIS PHENOMENA DOES NOT HOLD.

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