Does purity of chemical used to prepare 20X SSC, denaturation solution affect the results in the Southern Blot?

Kiran Kumar @Kiran-Kumar-128

17 October 2013 0 3K Report

I am using DIG-Labeling kit from ROCHE. After digestion gDNA is looking like smear in gel.

I am using the low grade chemicals (Nacl, Sodium citrate) for preparing the solutions.

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