Pseudomonas putida KT2440 could accumulate cadmium using protein transporters such as CadA, and CaDB. But after exporting the Cadmium inside, how do they maintain other metabolism and not become disrupted by the cadmium inside? Is there any specific mechanism so that the cadmium won't interrupt other metabolism pathways? Or do they have some mechanism to transform Cd(ii) into some less harmful form of Cadmium?
Hi ,
As per metabolic activity of microorganism (Say bacteria) heavy metal(esp., cad, chromium etc.,) will not take part in its metabolism. the only possibility is accumulation of those heavy metals in its cell wall or any other .. u can try out SDS page for the organism (you are using) or try out some biochemical changes owing to the Cad uptake with comparison to that of control experiments . this may help you to give some helpful picture about the changes in its metabolism. i hope there might be formation stress proteins in the bacterial cell wall.
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