I am unable to find an exact selection media where I can see the phenotype of adenine mutants from sick to cure after transformation on a plate
So, my question is about peptone composition and weather or not I can use YPD for selection of adenine mutant
my plasmid is URA3 marked and is having CUP1 promoter along with GFP
Peptone does not have uracil or adenine in it. Peptone is an enzymatic digest of casein protein so will only have amino acids in it. However yeast extract, which is also a component of YPD does have uracil and adenine. So you can not use YPD to select for your URA marker. Most labs use SD or SC with dropout mix for the selection.
Peptone does not have uracil or adenine in it. Peptone is an enzymatic digest of casein protein so will only have amino acids in it. However yeast extract, which is also a component of YPD does have uracil and adenine. So you can not use YPD to select for your URA marker. Most labs use SD or SC with dropout mix for the selection.
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