Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How to calculate enthalpy of formation of Polymers taking into account the terminal end groups?

I am trying to find methods for theoretical calculation of enthalpy of formation of Polymers. till now I have found the oligomeric method (Gaussian) which uses the extrapolation of results from...

05 February 2021 9,707 4 View

Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...

01 February 2021 5,809 3 View

How can we polymerize carbon to form a layer? Any easy method is there?

I want to polymerize the carbon to make a thin sheet. Is there any easy method to polymerize it?

10 December 2020 1,762 4 View

I am designing an UWB antenna..Please guide how to get wideband instead of multiband..?

By slotting patch and ground I am getting multiband not wideband.pls guide

09 November 2020 3,924 4 View

Can I run joint wald test for more than two regressions to interpret whether the intercepts are jointly equal to zero or not?

I have run 10 regressions using same model for a sample by diving the the sample on the basis of some criteria. The problem I am facing is that I can test the intercepts for individual sample...

25 September 2020 6,314 3 View

Is Cronbach's alfa value 0.5 acceptable ?

I am calculating the whistleblower policy disclosure score for 100 Listed firms in India. I took 31 questions under four categories and scored them 0, 1 & 2 on three-level a) 2 points: If...

09 June 2020 7,729 3 View

My Work Is On Information Retrieval Ranking Using Pso+Svm ,Using Trec Dataset Of Q/A ,But Some Good Journals Rejected Commenting Its Basic,Is It?

I did my implementation in python and represented my result score result of answers of the questions.I represented graph for all the evaluation parameters and confusion matrix,why i am not able to...

24 March 2020 4,235 3 View

What are the statistical method or procedure to construct Index?

I am constructing the whistleblower policy Disclosure Index for the top 100 listed companies in India. I have taken 31 attributes/questions/parameters under four major categories i.e. Board...

23 March 2020 6,571 3 View

How dialysis membrane can be prevented from rupturing during incubation on shaker?

I use beads inside dialysis membrane for metabolite extraction but membrane ruptured during shaking that allow cells to bind on beads. kindly suggest me the way out.

10 February 2020 3,669 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View