Help regarding free-floating immunohistochemistry on mouse spleen?

Hello fellow researchers I am trying to stain cryostat-sectioned spleen tissue, using free-floating chromogenic immunohistochemistry. Unfortunately, I'm not able to detect any staining (MHC class...

13 November 2020 6,884 3 View

How much plant powder do I need for different concentration of two extracts (Aqueous and Ethanolic extract)?

Supposed I need three different concentration for two plant extracts (namely: Aqueous and Ethanolic extract), starting at 50mg/ml, then 100mg/ml then 150mg/ml. In what particular weight of plant...

31 March 2019 2,775 5 View

Does anyone have any experience running ERIC-PCR?

11 May 2016 526 39 View

How do I get the electrode surface area for CR2032?

I am trying to compute the Lithium-ion diffusion coefficient (DLi) based on the equation attached[1]. So how do I get the A; electrode surface? and also how to calculate the dE/dx part of the...

13 March 2016 4,458 3 View

Any papers that have specific gases and its composition in carbonization of coconut shell?

carbonization, coconut shell, gases, carbonizer

24 February 2016 2,412 3 View

Dissertation query, Q - method inquiry. currently running a pilot study and am falling short on the analysis. All info welcome?

Hi, I'm an Undergraduate student at the University of Chichester and I'm currently writing my dissertation. I have all my statements and my question, i have knowledge of exel and spss software...

04 January 2016 3,364 4 View

What factors affect the electrode polarization for lithium-ion batteries?

Surface area, particle size? What other possible factors reduces/increases Electrode Polarization? And how does Electrode Polarization affect affect rate-capability and battery performance?

08 December 2015 2,303 6 View

Who will assist me in developing a new theoretical concept on our oceans' formation - ?

Hello and thank you for welcoming me here. I am thrilled to announce that I seem to have developed an in itself seemingly "water-proof" raw theory on the formation of our (and any) planet's...

01 September 2015 2,035 6 View

Did someone try to ablate metallic cerium?

I want to ablate metallic cerium but i'm concerned about the inflammable nature of cerium. Any suggestions? Thank you!

24 August 2014 1,285 2 View

How do I perform an ELISA validation?

I am performing a (partial) validation of commercially available ELISA kit. I am using the kit on a previously untested matrix, so I wish to validate the assays Accuracy, intra-assay Precision and...

02 April 2014 8,905 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View