Does Methanol fixation of cells require any antigen retrieval for Imnunofluorescent labeling of surface receptors?

09 September 2012 1 479 Report

For labeling surface receptors methanol fixation or formaldehyde fixation is better?

Yes methanol fixation usually does not require any antigen retireval step. The rehydration in water based buffers in immunofluorescent staining does it.

Secondly, cold methanol is surely better for surface receptors than formaldehyde, but cold acetone can also used

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