Most of the conjugates are captured in the column when a Zeba desalt spin column from Thermo was employed for separation.
I can find in NEB website that they are resistant to streptomycin (https://www.neb.com/-/media/nebus/files/brochures/compcell_brochure.pdf). But these cells available on NEB are derivative of DH10...
31 December 2017 5,006 1 View
I need to test the concentration- dependant uptake of nanoparticles. Can anyone give different options for hydroponic medium (both commercially available and composition for medium)
06 July 2016 3,981 2 View
For labeling surface receptors methanol fixation or formaldehyde fixation is better?
08 September 2012 431 1 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Thanks
22 July 2024 1,143 3 View
Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...
21 July 2024 2,476 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View