Does melon gel allow co-purification of IgM and IgA or IgG only?

05 May 2015 1 9K Report

I'm interested in extracting total antibodies from serum. Pierce's Melon Gel product mentions briefly that it binds non-immunoglobulins so that you get the immunoglobulins out in the flow-through, but they then go on to talk about IgG only. Does anybody know if you can also get IgM and IgA out with this? https://www.lifetechnologies.com/order/catalog/product/45212

Michał Skowicki

As I know it's only for IgG, for IgM you can use Mannan Binding Protein Agarose:

https://www.lifetechnologies.com/order/catalog/product/22212

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