I am doing immunolabeling of plant material with cell wall specific AB and would like to remove the epitope with an enzymatic pre treatment as a control.
2. Method of LR polymerization; its influence on the epitope
3. Hydrodynamic radius of the enzyme used; penetration into the section will be influenced by the size
LR White, being hydrophilic, is much more imumunodetection friendly but fundamental issues related to integrity and accessibility to the antigenic epitope will still need to be considered.
Without details, this is about as much as one can assist you.