I am working with Camelina sativa, utilizing Agrobacterium-mediated transformation and the floral dip method. Following transformation, I used kanamycin as a selection marker to differentiate transformed from non-transformed Camelina plants. I tested various kanamycin concentrations: ,25 ug/ml,50 μg/ml, 100 μg/ml, 200 μg/ml, and 400 μg/ml.

After stratification (2 days at 4°C), light treatment (6 hours), dark treatment (2 days), and subsequent light exposure (5 days), I observed no noticeable differences among seedlings on plates with varying kanamycin concentrations. Notably, all seedlings developed green cotyledons.

Does this indicate that kanamycin is ineffective as a selection marker for Camelina sativa?