Krishna Nandakumar

3 Questions 1 Answers 0 Followers

Questions related from Krishna Nandakumar

Does Kanamycin be used as a reliable selection marker for distinguishing transformed camelina sativa from non transformed camelina?

I am working with Camelina sativa, utilizing Agrobacterium-mediated transformation and the floral dip method. Following transformation, I used kanamycin as a selection marker to differentiate...

09 September 2024 6,405 1 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

03 August 2024 1,542 3 View

Why is colony PCR not working ?

I've been doing colony PCR for last few days but haven't seen any bands on the gel. I've tried both positive and negative controls, but I'm still not getting any bands. Here's my procedure: I use...

08 June 2024 293 2 View