Can anyone tell me the names of International or National Journals (Engineering & Management) with free of cost publishing fee ?

08 September 2016 3,959 2 View

Please tell me that Journals which are indexed on "Researcher ID or Endnote" of Thomson Reuters are good or not ?

02 March 2016 9,641 2 View

Is IAEME journal fake or not ?

IAEME publishing group has many journals and they are also showing that it is indexed on Thomson Reuters with unique researcher ID having more than 2000 papers published on that ID. I have a doubt...

01 February 2016 5,928 18 View

Does anyone know how to calculate the cofficient of thermal expansion of concrete or steel?

Please share some methods or process to find it.

31 December 2015 8,949 2 View

How can we produce a fire resistant concrete?

Does anyone have some publications on this topic? Please share

31 December 2015 1,803 2 View

How can we construct round skyscrapers?

Please provide relevant information.

31 December 2015 6,365 0 View

Is Fiber reinforced concrete matrix available in India and where ?

Please share your details.

31 December 2015 3,694 1 View

Does anybody know how can I measure the field capacity of a specific soil in the simplest way?

Suggest some easiest techniques.

31 December 2015 3,878 0 View

Is State Goverment University better to pursue PhD than a Deemed University ?

Give me some vital difference points.

31 December 2015 790 0 View

What are the techniquies to evaluate the energy saving potential of a building?

Is there any particular software for that?

31 December 2015 8,642 0 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Why do I have trouble with cloning duplicate gene in the same plasmid?

Hello! I am trying to generate a construct in this format: seqA - seqB - linker - seqC - seqB into pSF-CMV-Ub-Puro-Ascl plasmid 0.5kb 2kb 66 0.25kb 2kb I have tried many...

15 February 2021 7,118 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Quality of antibodies from Creative Biolabs?

It seems Creative Biolabs has everything and so many versions of antibodies not owned or sold by any other company. How reliable are their products? I am looking for a clone where potentially...

08 February 2021 2,167 3 View