Does human cloning actually work?

More Piyush Sharma's questions See All

Can anyone tell me the names of International or National Journals (Engineering & Management) with free of cost publishing fee ?

I am interested in engineering and management journals !

08 September 2016 4,109 2 View

Please tell me that Journals which are indexed on "Researcher ID or Endnote" of Thomson Reuters are good or not ?

I have found some journals which are NOT included in the official list of Thomson Reuters but these journals have a link on their websites, that link is of "Thomson Reuters Researcher ID" and on...

02 March 2016 9,797 2 View

Is IAEME journal fake or not ?

IAEME publishing group has many journals and they are also showing that it is indexed on Thomson Reuters with unique researcher ID having more than 2000 papers published on that ID. I have a doubt...

01 February 2016 6,282 18 View

Is Fiber reinforced concrete matrix available in India and where ?

Please share your details.

31 December 2015 3,821 1 View

How can we produce a fire resistant concrete?

Does anyone have some publications on this topic? Please share

31 December 2015 1,944 2 View

What are the techniquies to evaluate the energy saving potential of a building?

Is there any particular software for that?

31 December 2015 8,797 0 View

Is State Goverment University better to pursue PhD than a Deemed University ?

Give me some vital difference points.

31 December 2015 928 0 View

Does anyone know how to calculate the cofficient of thermal expansion of concrete or steel?

Please share some methods or process to find it.

31 December 2015 9,104 2 View

Does anybody know how can I measure the field capacity of a specific soil in the simplest way?

Suggest some easiest techniques.

31 December 2015 4,024 0 View

How can we construct round skyscrapers?

Please provide relevant information.

31 December 2015 6,501 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Cloning of flaviviruses is difficult i have noticed that cells loose their transformation ability after cloning fragments inside vector any advice?

As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...

11 July 2024 4,284 0 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

How can I verify if an academic journal is a legitimate publication or a cloned version?

This question seeks to address the growing concern of cloned academic journals, which are fraudulent duplicates of legitimate publications. It aims to guide researchers, scholars, and academics on...

10 July 2024 5,966 2 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

I need help with cloning a 200 bp insert and a 4,7 Kb pEGFP-N1 vector. How can I do it?

I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...

09 July 2024 4,277 1 View

Ileana de la Fuente

there is this paper, they achieved to modify human embrios using the CRISP/Cas9 system

http://www.nature.com/news/chinese-scientists-genetically-modify-human-embryos-1.17378

Sabine Strehl

Everybody should also read the accompanying article:

http://www.nature.com/news/don-t-edit-the-human-germ-line-1.17111