Dear All

I'm looking at the amplex red reaction with HRP to generate the fluorescent product resorufin. People are not very good at fully balancing the reaction schemes... I try to understand what happens to the carbonyl group. Based on balancing the whole reaction, including H2O2 conversion to water, it seems like acetic acid is also formed. Is this correct?

Here's one example of the scheme:

https://www.biotek.com/resources/application-notes/horseradish-peroxidase-hrp-determination-using-amplex-red-and-synergy-ht-multi-mode-microplate-reader/