yes it continues to fluoresce and give signal.

Thanks Murat.

Can you suggest me some literature for reference ?

I do not have reference actually, but this is my experience. I have tagged a protein with GFP, the protein formed aggregates, due to misfolding but I still got GFP signal.

Meantime you worry about misfolding could diminish signal of GFP, keep in mind that the chemical you use during process might have affected the fluorescence by bleaching it. Check the chemical's properties.

I had a briefly experience working with C. elegans and one of the experiments my colleagues were working that time, was related to measure the accumulation of certain proteins tagged with GFP.

So I can say it works when the protein is aggregated. Try looking at some papers involving C. elegans, specially the work of Dr. Blackwell

http://www.joslin.org/diabetes-research/t_keith_blackwell.html

Ajai, I used an aggregation-prone polyglutamine tract fused to GFP, which forms fluorescent aggregates. See my first-author publication on my profile.

Also, see the work of Richard Morimoto's lab at Northwestern University.

Example: http://groups.molbiosci.northwestern.edu/morimoto/research/Publications/sdarticle.pdf

and here: http://www.jneurosci.org/content/26/29/7597.full

Thank you all...

It was a great help :)