Gene expert PCR techniques has been explored for diagnosis of MycoBacterium Tuberculosis ,any relevance for diagnosis of Mycobacterium Leprae ,any ongoing trial available.?
People in industrial anticancer drugs manufactory are continuously expose to anticancer drugs though aerosol or skin contact, etc, does this lead to cancer of any sort in near or distant future?
07 August 2018 2,471 0 View
i have a clinical presentation on benign anal lesions and how they can be treated ,most recent innovations .help please.
06 July 2018 7,988 0 View
Journal Publication ,these days seems costly for the developing nations and therefore cannot afford the costly Publication fees. i therefore use this medium to get information to help researchers...
03 April 2018 2,004 2 View
Robot have been developed to replace human effort for its daily activities and Jobs, which area of human activity is aggressively been replaced by artificial robot intelligence. thank...
03 April 2018 612 1 View
Physicians in Poor countries have been coming across evolutionary and new diseases but cannot publish on good platforms due to high cost ,The full salaries of low income countries are quarter...
03 April 2018 5,655 0 View
Hydrogen (proton energy has been evolving in the Scandinavia developed from water .Can this be incorporated into self powered medical equipments?
02 March 2018 6,347 3 View
many energy and technology companies have not incorporate solar energy development to get more from the African,Caribbean consumption market despite high sunlight abundance .why
02 March 2018 3,254 6 View
There are many drugs used to treat HIV ,yet preventive measure are not in full gear in combating the increase ,is there any immunization for HIV yet.
31 December 2017 4,573 0 View
We believe that the detection of Cancer is still slow compare to several other disease.Any idea on research and laboratory technique that can combine screening of multiple cancer oncogene or...
31 December 2017 8,435 1 View
Many Institutions in Africa lack skilled experience physicians for endoscopy ,especially enteric endoscopy, WE HAVE so much suspected Ulcer patients in North-East Nigeria ,this may be the same for...
10 November 2017 6,983 6 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View