in cell culture experiments...Does exogenous oxidized glutathione (GSSG) protect cancer cells? Is it converted to the reduced form GSH once it's taking up by the cell to protect the cell? i know that both of them is forming the other and the amount of formation of certain form depends on the status of the cell. I'm working on cancer cell lines and when i used both forms separately to see their effect on cell survival, both of them shows cell protection with no cytotoxicity. I'm also using a cytotoxic drug ( with electrophilic structure) that possibly reacting with the thiol group of the reduced form GSH and becomes inactivated when i combine it too with the oxidized form GSSG it also shows no toxicity to the cells.. Is it possible that GSSG converted to GSH and then react with my drug.. Can someone please help me find an explanation to this result?
Some authors explain such effects due to a complex change in the activity of telomerase. Please see the link in the Attachment when using NAC. But the main thing that became clear to us from such experiments. The data we obtain in vitro experiments very often contradict the data that we observe in experiments in vivo. Please compare the results obtained in 2D and 3D experiments, as well as in vitro and in vivo. Based on the comparison, think about the key mechanisms.
I forgot to mention the test I used for both is MTS proliferation assay.
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