Can someone provide clarification on Total Ca estimation?

The procedure for total Ca estimation says that a known quantity of sample mixture was dissolved using conc. HCl & diluted to make up to 100 ml with H2O. From this solution, 20 ml were...

07 August 2015 3,833 16 View

How might I calculate the activity of immobilized cellulase?

06 July 2015 153 6 View

If a solid powder sample contains both calcium carbonate and calcium hydroxide, how can we quantify each in percentage?

Present methods are used to characterize as cations and anions, but i would like to know how to characterize calcium carbonate and calcium hydroxide as a compound.

01 February 2015 3,965 11 View

How can an immobilized Enzyme separated from the industrial effluent be reused?

Normally immobilization is mainly done to improve stabilization and for reuse, how immobilized enzyme separated from an industrial effluent?

10 November 2012 4,289 11 View

How does Dinitrosalicylic Acid stops the enzyme-substrate reaction, in enzyme assay?

Normally it is said that dns is added to stop the reaction in enzyme assay, what happens to the enzyme.

08 September 2012 2,959 8 View

Why does DNS precipitate when added with sodium acetate buffer pH 4.8, while doing enzyme assay for finding glucose concentration?

Cellulase enzyme assay using DNS

07 August 2012 1,755 4 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View