We are looking to quantify PAH content of plant material grown in various substrates. References vary, but some suggest extraction of fresh material, some freeze dried and some oven dried (50-70oC). Due to other analyses to be performed, it would be most convenient to oven dry, however, I am concerned that oven drying may volatilise some of the PAHs in the tissue. Has anyone compared the sampling and preperation methods (fresh, freeze and oven-dry) against one another? Thank you!
Grzegorz Boczkaj
YES!
This compounds are slightly volatile and you will obtain lower recovery using 50 or 70 deg.C.
You can minimize this probelm using 2-3 internal standards (best deuterated PAHs, addition before drying), which will have comparable recovery.
regards,
G
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