What is the recommended/optimal time for resting mouse splenocytes after thawing?

This is my first time working with mouse cells. We isolated and froze down in 90% FCS and DMSO. I would like to retrieve them and do immunophenotyping and intracellular cytokine staining

03 January 2021 6,399 1 View

Can CMOS and CCD images sensors be used as microphones?

I was thinking about Msps and Gsps image sensors in different environments. If a pair of identical image sensors - one exposed to air, gases, plasmas, or partial vacuum - and one in a good...

21 December 2020 9,747 2 View

Is there a tool to find the closest species with an assembled genome for any given species?

As more and more genomes are being sequenced the likelihood of having a close relative being sequenced is rising. So lets say I have a non-model species Chela cachius and I want to find the...

25 November 2020 6,267 4 View

Where can I find a longterm global mean EVI/NDVI dataset?

I am looking for a longterm (10+ years) dataset of mean EVI/NDVI or annual maximum EVI/NDVI and similar. Basically, I am trying to avoid downloading tons of images and doing the averaging myself,...

28 September 2020 8,374 2 View

What would be the criteria for an expression vector designed for S. cerevisiae to also work in S. pombe?

I am interested in using a S. cerevisiae expression vector such as the pYES2-DEST52 plasmid for transgene expression in S. pombe. This plasmid has the GAL1 promotor, so I would like to make sure...

18 September 2020 7,460 2 View

Can we infuse drug along with sedative?

I would like to infuse rats with drugs of our interest. However, we also need to keep animal under sedation by administrating pentobarbitol via jugular vein with the help of syringe pump since we...

15 September 2020 7,501 5 View

What is a suitable solvent for extraction of arsenic?

Hi. I'm doing some work on arsenic speciation. I am following a paper titled, "SPECIATION OF ARSENIC IN A CONTAMINATED SOIL BY SOLVENT EXTRACTION." which calls for the use of chloroform or benzene...

06 September 2020 6,351 4 View

Ideal saline volume for intravenous injection in rats ?

Hello everyone, my interest is to understand the molecular mechanisms involved in anaphylactic shock using Wistar rats. While i inject non-allergic naive rats with iv bolus (1 mg ova in 1 ml),...

27 August 2020 9,444 2 View

Tissue Digestion with proteinase k gives "invisible" globule"?

I'm digesting a few different mammalian tissues with a spin kit and proteinase k. Some tissues (esp. testis and pituitary gland) look fully digested and clear. But once the lysis buffer is added,...

09 August 2020 4,443 2 View

Can I use a spin column kit to extract DNA from bull testis?

I'm extracting DNA from various bull tissues and there are some that don't work with a spin column kit, like brain tissue that contains a lot of lipids. For those, we do Phenol-chloroform...

05 August 2020 2,126 2 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View