Sure, Chinmay Sen, every function in the cell should start as biochemical reaction. simply, genes also chemical compounds and all the processes: transcription, splicing, transfering, translation and translocation etc.... all chemical reactions.
So alternative splicing is defently affected with the chemical changes.
Alternate splicing, or or differential splicing, is a regulated process during gene expression that results in a single gene coding for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA ( mRNA) produced from that gene. Alternate splicing is a mechanism used by many protein classes, including muscle - related genes, hormones, and transcription factors. All these processes involve biochemical mechanisms.
Usually splicing and overlapping genes would not be in the course of Biochemistry, but would be in Molecular Biology. Overlapping genes (actually as well as splicing) might also be more specifically Genetics area. The biochemical steps of course are needed for e.g. splicing to occur, but if you are looking for a course to take to learn about those to subjects: go for Molecular Biology (or maybe Genetics).
As someone already explained splicing, I won't touch it. But with overlapping genes - it depends what organism are you talking about and what is exactly in your interest. In general - this is when the same block of mRNA (or DNA ) can produce more than one protein. One of the possibilities is when the frame is changed. e.g. protein 1 is encoded by 123 456 789 ... nucleotides, while from the same nucleotides protein 2 would be translated but in different frame, starting as e.g.. 234 567 8910 .. which would produce different amino acids. There are many different ways for overlapping genes to be expressed, therefore it really depends what is your exact question.
Molecular biology is a major branch of biochemistry, by default you can find an overlapping between the two sciences in many topics including alternative splicing phenomenon.
many biochemistry Books and references mention the concept of alternative splicing in different contexts. while in molecular biology text books you can find more concrete, organized and comprehensive details of the process mechanism and related issues.