I wish to study the effect of autophagy inhibition on lipid accumulation in HepG2 cells when treated with free fatty acids (palmitic acid and oleic acid). I have observed that HepG2 cells when treated with free fatty acids have increased levels of lipid accumulation. My treatment compound reduces this lipid accumulation. It also induces autophagy.
However, when I try to inhibit autophagy with chloroquine and 3-MA, lipid accumulation reduces even further. Inhibition of autophagy significantly reduces lipid accumulation in my experiments. Whereas, available literature suggests inhibition and impairment in autophagy leads to an increase in lipid accumulation.
Can someone guide me through this discrepancy in data? Looking forward to your kind inputs. Thank you in advance.
I think it is quite common in adipocytes. Inhibition of autophagy using BafA1, CQ or similar at early days of maturation seems to have a strong effect on trout adipocyte differentiation "blocking" both, phenotype changes and gene expression. It has also been reported in mammals.
Check out this article: Article The autophagy response during adipogenesis of primary cultur...
'I would assume something similar may occur in hepatocytes. I hope this helps :)
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