I would like to perform a RT-PCR analysis for assessing these genes expression in C. raciborskii under different conditions.
What volume of Chloroplatinic acid is required to form 10 ml of a solution in which it is 16 mM? The density of Chloroplatinic acid is 2.43 g ml-1, and its molecular weight is 409.81. I really...
18 February 2024 7,908 2 View
Does anyone know of any software/websites to use AI in the field of medical statistics? I have a database of 2500 patients to analyse and it would be useful to be able to exploit AI.
19 June 2023 6,904 3 View
I wonder either my antimicrobial susceptibility test on Muller-Hinton agar is contaminated or a subpopulation of my bacteria is resistant to those antibiotics. The thing is, when I was measuring...
11 April 2023 2,514 2 View
I'm interested in transforming a Staphylococcus aureus strain with a GFP-expressing plasmid. Is it possible to do it with the Calcium Chloride technique?
01 April 2023 4,421 0 View
Im doing my research about diarrheagenic E. coli and I wonder if something like that would be possible. Thanks.
07 March 2023 5,563 11 View
I am mapping residual stresses in Abaqus and I wonder if I need to add a step to find equilibrium before loading the model when I use the SIGINI subroutine. Thank you for your help.
05 March 2023 8,479 1 View
I am working with a recombinant protein expressed in BL21-SI. The protein is expressed all the time, even if the induction is not carried out with NaCl, but this is not a problem, I just need...
04 January 2023 7,124 3 View
I wonder either a table like that exists, or a reference method to determine every functional group in a FTIR spectra. Thanks.
04 November 2022 6,740 2 View
I am trying to model fatigue crack propagation using XFEM in Abaqus and I observed that I could create three types of steps for this task: static-general, dynamic-implicit and direct-cyclic. What...
22 October 2022 7,691 4 View
I am looking for information about non-chemical control methods of invasive species in Europe. Thanks for your help.
07 February 2022 736 4 View
Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...
06 August 2024 5,023 1 View
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
I want to prepare 0.05 M bicarbonate carbonate, pH 9.6 as coating buffer for ELISA. Could anyone help me with right recipe, procedure, and or reference in the preparation? Thank you in advance.
10 May 2024 4,906 4 View
How can I calculate the DIC value in natural water if I have concentrations of alkalinity, bicarbonate, pH, water temperature?
27 March 2024 1,485 0 View
In the pore water of a long-cultivated paddy soil with high CEC (smectitic clay dominance), high organic matter and a clay pan (physical constraint) below 15 cm depth, can the total anion...
23 January 2024 10,037 2 View
"I recently graduated from the School of Botany at the University of Malakand, Chakdara, Pakistan, specializing in Ecology with a focus on Crop Stress Physiology. I have practical experience in...
23 January 2024 8,363 4 View
Resuscitation drugs become ineffective during severe metabolic acidosis ...why can't bicarbonate be a part of ACLS protocol
22 January 2024 5,099 3 View
Hi all I'm doing Sandwich ELISA , I wanted to coat the antibody to the wells , So I have many questions: 1: Coating buffer ( which is more accurate) bicarbonate -carbonate buffer: at 100mM or...
28 November 2023 4,290 0 View
Our process involves generating plasma with Ar and Hydrogen gases, followed by the deposition of Hydrogenated Carbon.
27 November 2023 9,443 2 View
My whole lysate proteins were prepared in 8M Urea buffer +2M Thio-Urea+ 4% CHAPS. In preparation for LC-MS/MS analysis, the samples need to undergo desalting and be switched to Ammonium...
10 August 2023 7,188 2 View