Does anyone knows what is the mechanism of initiation with 2-oxoglutaric acid in Acrylamide polymerization ?

More Antoine Beziau's questions See All

How can I improve my plasmid DNA yield using CopyCutter EPI400 for a toxic gene?

I am trying to clone a large (11kb) and toxic gene in an expression vector. Therefore, I am using the EPI400 CopyCutter strain that is maintaining the plasmid at a low copy number to allow...

09 April 2024 2,861 3 View

What cells am I retrieving from my cardiomyocyte isolation process ?

I have tried isolating ventricular cardiomyocyte by perfusing type II collagenase through adult mice hearts. When looking at the extracted cells under 20X magnification, I don't see the typical...

21 March 2024 3,725 1 View

What is the meaning of negative coefficients of partial determination (i.e., partial R-squared) in GLM?

Dear Community, I am interested in reporting the relative importance of my predictors in a GLM (Gamma distribution, log link). In other words, among significant predictors, I would like to know...

16 November 2023 8,093 3 View

What indicator should I use to observe calcium oscillation in lung tissues ?

After making PCLS (Precision Cut Lung Slices), I want to observe the slices under a fluorescent microscope and quantify the calcium ion release during bronchoconstriction. To do so, I used two...

26 October 2023 6,952 0 View

AAV Extraction from supernatant ?

I am trying to perform AAV long-read sequencing using the PacBio protocol. The protocol requires 1.5x10e12 Vg input to use 100uL and get 1ug DNA at the end of extraction, in order to get around...

18 October 2023 690 1 View

What are the effects of the stock position on the lead time for supplying critical raw materials in the just-in-time circular economy business model?

The basic notions of the question are those meeting the definitions of circular economy, and critical raw materials of the European Union.

20 June 2023 10,020 0 View

How do I use ODB file as an initial field for a dynamic explicit simulation on Abaqus?

Hi everyone, I'm simulating the impact of a ball on a cylindrical beam embedded on one side and free on the other side at high temperature on Abaqus. A high temperature is imposed on the top...

21 May 2023 3,972 0 View

How can poly(methyl) methacrylate PMMA be dissolved in acetone?

I would like to make a colored PMMA based ink for an aerosol inkjet printing. For this, I was able to find the printing settings and some on the ink...

19 April 2023 613 0 View

How to reliably and quickly determine if an emulsion is a W/O emulsion ?

How to reliably and quickly determine whether an emulsion is a W/O emulsion (or W/si) or a W/O emulsion? Can the measurement of pH (or the impossibility of measuring pH) be a sufficient method?

11 April 2023 4,349 2 View

How to improve abnormally slow computation speed in ANSYS ?

Hi ! I'm trying to simulate the lorentz force on an actuator between two magnets. This is a transient solver simulation. My 3D model is ridiculously slow and I don't understand why... Here is...

11 April 2023 1,740 4 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Help. How can I fix my T7 reaction problems?

I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...

16 July 2024 4,604 0 View

Can anyone help in getting software related to gel scoring of silver stained PAGE for diversity study?

I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...

12 July 2024 7,145 2 View

Troubleshooting Slow Migration and Poor Separation in tRNA Electrophoresis?

Hi everyone, I've been trying to replicate the protocol described in this paper: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22736012/ for separating aminoacyl-tRNAs from deacylated tRNAs...

14 May 2024 7,257 3 View

Size acrylamide pores?

Good evening, I was wondering what is the size of Acrylamide/Bis-Acrylamide in a PAGE 4%/8%. I'm asking because after the run I found that some of the sample stayed in the well

04 May 2024 1,566 3 View

Cloudy background of polyacrylamide gel after de staining, a confusing problem?

Hello. My polyacrylamide gel becomes cloudy after de staining with a solution consist of (50% miliQ water + 40% methanol + 10% acetic acid) and the background of my gel does not become completely...

02 May 2024 895 2 View

DNA PAGE - Why are my Bands and Ladder smeared/Not Clear?

I am running a DNA PAGE after PCR (samples 6-15 are run in duplicate with the second sample digested) to determine serotonin genotypes. The ladder (well 1) is on the far right of the attached...

01 May 2024 5,427 2 View

What is the best solvent to precipitate a lactide macromonomer?

I'm trying to remove acrylamide and lactide from the medium that didn't react to form the macromonomer

29 April 2024 4,903 1 View

PAGE with urea for miRNA integrity?

Hello, I'm trying to elaborate a denaturing 12% polyacrylamide gel (19:1) with urea 8M in order to visualize the presence of plasma total miRNAs, I'm using TBE buffer, my extraction (Trizol)...

22 April 2024 1,740 3 View

Can more than one TetraHis antibody bind the long His tag?

Hello. TetraHis antibodies recognise 4xHis-tag in the protein. What if I have a long His-tag (e.g. 10xHis-tag) and I perform ELISA or Western Blot based on native PAGE? Is there a chance that two...

15 April 2024 6,959 1 View