Any ideas? or is it just because CH5alfa is considered non pathogenic.
Hello Franz, seems like your positive control of LPS does not seem to be working either? I am assuming this is mean fluorescence intensity data plotted after conducting flow cytometry to analyze expression of the markers indicated. can you please provide more details regarding your experiment? Like for instance when did you measure the surface markers (i.e how long after incubation), did you use isotype controls in determining relative expression etc.?
What is your control? Would you be able to write the experimental details and represent the figure & legend in English please? I think you are not catching the dynamic range. Your control indicates some artifacts. Try to think about some interference. Let's know the experimental details.
Thank you very much for your answer, and sorry for my late answer.
Dear Chirajyoti my control is Macrophage without stimulation.
Or in fact, it could be still THP-1 or even Monocytes, i´m note sure.
Dear Gayathri for the bacterial infection i just wait 4 hours, could be that the reason that i don't see any response even in the positive control???
Thanks you very much for your help
Dear Gayathri i didn't use isotype controls, just cells without stain.
Dear Franz,
I always use E. coli DH5alfa as a negative control in my experiments of adhesión and invasion of culture cells because this is considered non pathogenic and is not abe to adhere to cells. I work with Caco and RTG2 cells.
Dear Alicia,
Entonces tu crees que la explicación mas adecuada a estos resultados sería la patogencidad de E coli mas que el tiempo de incubación? Te lo pregunto para poder enfocar de mejor forma la discusión de estos datos.
Muchas gracias por tu ayuda
Estimado Franz,
Efectivamente, en mi opinión la explicación a tus resultados estaría más con la falta de patogenicidad de la cepa de Escherichia coli utilizada. Suerte!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques