02 February 2020 2 9K Report

I am looking at neurogenesis by injecting mice with EdU, and treating for a month. After sacrifice, I stained brain slices with either NEUN or DCX and DAPI. When I visualized the samples, I saw that there were some DCX positive cells without any EdU. I saw that neurons only express DCX for around 4 weeks, so with the 1 month treatment time after EdU injection, there shouldn't be any DCX positive cells without EdU. Does anyone have any idea what could be going on?

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