The BY-2 cells are non green cells and even when they are under continuous light, don't change their color. Can changing hormone in their media can make a difference? I worked with other cell lines like Arabidopsis, they are green when light is present but, I am not sure about the duration of the time/exact process needed for BY-2 cells to let the other pigments act. Please let me know if you have such experience or any paper related.
Thanks in advance,
Hi Mahbobeh,
tobacco BY-2 cells are derived from an induced callus tissue of Nicotiana tabacum cv. BY-2 (a.k.a. cultivar Bright Yellow - 2). It was published in 1972 by Kato et al. For first characterizations you may read the link below. The original cultivar is by default, as the name tells, of a bright yellow color. So even if you would induce a callus and breed a plant out of it, it would still be bright yellow, not green as other tobacco plants.
http://www.sciencedirect.com/science/article/pii/S0074769608624523
Hi Falco,
Thank you so much. Now it makes sense for me. I didn't notice to its name!
Best,
Because the original BY-2 cells are derived from Nicotiana tabacum cv. BY-2 (a.k.a. cultivar Bright Yellow - 2)
You should also keep in mind that many (rather most) of the call grown in solid or liquid culture don't look green because the chloroplast development is lacking in them. Many, but not all, could produce plants that would be perfectly normal green.
AM looking for N.tabacum cv Bright yellow seeds.I need the whole plant.
Question: they are bright yellow, do they have chlorophyll/chloroplasts? If not, how do they do photosynthesis?
they don't do photosynthesis as they don't have chloroplsts. instead, they have proplastids
@Mohammad,
If they don't do photosynthesis, how can they survive as a plant?
Those plastids that contain chlorophyll can carry out photosynthesis.
@yuan-yeu yau
TBY-2 cells are a plant cell line cultured in vitro in adequate medium containing nutrients, sucrose, auxin, thiamine etc..
they are heterotrophic cells, do not become green and do not do photosynthesis
@ Mohammad Issawi,
Thanks for your message.
Yes, I know that. But, What I meant was: can they grow on the field as a normal plant? Do people grow them out and harvest their seeds for future growing? Or do they have to be reporduced through in vitro, and if so (through in vitro), how was the very first batch of cells coming from?
Sorta out of left field here for the thread bump, but can anyone suggest a source for BY-2 cells? Thanks!
@ Evan Labrant,
Yes, we were just talking about this a few days back on RG (see below link). RIKEN in Japan has the cell line (BY-2).
See (copy-and-paste): https://www.researchgate.net/post/Where_can_I_purchase_Nicotiana_Tabacum_BY-2_cells
- Badges
- Science topic
- Similar topics
- Computer Communications (Networks)
- Simulators