Does anyone know why a 60:40 methanol: water mixture is used as solvent in the extraction of polyphenols from olive oil? Many thanks!

More Lucienne Gatt's questions See All

Is there a single cell transcriptome dataset for mouse Th2 cells?

Does anyone know of a freely accessible single cell mRNA profiling dataset for mouse Th2 cells? I came across various RNA Seq data for naïve T cells, Th1, Tregs...but not for Th2. That would help...

27 January 2021 7,784 2 View

Funnel plot indicates heterogeneity between studies - exclude those that cause asymmetry?

Dear research community! I am currently conducting a meta-analysis for my MSc thesis. My funnel plot is asymmetrical and indicates heterogeneity between studies rather than publication bias....

08 August 2020 1,220 3 View

Does anyone have a copy of Burger 1994 in Reviews in Environmental Toxicology and Applied Pharmacology ?

Hi all I'm looking for the following publication. I've not been able to come across it online. If anyone has a copy I'd really appreciate it! Burger, J. 1994. Metals in avian feathers:...

12 January 2020 2,044 4 View

Can blood smears be fixed with abs. ethanol instead of methanol?

I need to carry out the blood staining on the field, which is a remote island without lab facilities, and have to transport the reagents with me by plane and boat. I can't safely transport...

21 February 2017 9,755 0 View

Why is olive oil first diluted in hexane before liquid-liquid extraction of phenolic compounds?

When performing liquid-liquid extraction to extract phenols, the oil is first diluted in hexane. Why is this?

06 April 2016 4,713 2 View

What software is there to quantify eggshell pattern parameters through digital photography?

I want to quantify and compare egg phenotype, using pattern (blotchiness etc.) as a variable. Is there any software to obtain such values from digital images? Alternatively, do you know of other...

01 November 2014 5,260 3 View

Would anyone know of any research on how climate influences the performance of green roofs?

If we take the Mediterranean, its not one distinct climate it varies greatly from east to west and north to south. The north is more temperate-like with rainfall more dispersed throughout the year...

16 March 2014 6,933 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

AGILENT HPLC 1100 SERIES , any expert available please?

AGILENT HPLC 1100 SERIES operation assistance needed.

25 July 2024 10,045 2 View

Catherine M G C Renard

Because it works? Fun apart, as a rule polyphenols are most soluble in aqueous solvents (water : methanol , water : ethanol, and best water : acetone, with about 50 - 30% water). Of course this means it is not at all selective. A theoretical chemist could probably expain why, I just observed.

Sanjib Saha

For polyphenol extraction 80 to 100% acetone aqueous solvent is best solvent I have seen so far.