I am working on some ceramic samples with unexplained surface colour which I think may be linked to surface roughness. Can anybody explain?

We are working on some sintered ceramic samples that have different surface colours. Energy dispersive X-ray spectroscopy does not yield surface compositions that would explain a change in colour....

12 December 2019 3,778 3 View

Is this a unbalanced nested model? How should i go about analyzing this kind of design?

I am analyzing data from a field study. The main goal of this experiment is to understand what intervention can help increase students verbal reasoning. There are 4 kinds of interventions (say...

30 July 2019 7,037 5 View

What kind of factor analysis should i do on a questionnaire that has both ordinal and dichotomous items?

I am developing a scale that contains both ordinal and dichotomous items that are hypothesized to be affected by the same latent construct. For dichotomous items, tetrachoric correlation matrix...

08 May 2019 9,794 2 View

Can you reliably impute entirely missing surveys from a longitudinal study?

My colleagues and I have delivered a 4-wave survey (day 1, day 30, day 60, day 90), which makes use of multiple psychometric measures. We have the complete data for each survey collected, but also...

04 June 2018 8,786 5 View

Correlations and PCA for use in a SDM - use of background points?

Hello, I have a series of environmental data layers in r that I am trying to do a PCA on to reduce dimensionality for my SDM (species distribution model). I am attempting to do this in R in...

07 December 2017 8,591 7 View

When using RDA, why does my explanatory matrix explain a large amount of variation in my response matrix, but is not significant?

15 March 2017 5,983 3 View

Can anyone point me to a reliable NCAM antibody to use on rat tissues for IHC?

I'm willing to do fluorescence or DAB. Rat muscle, snap frozen, 10um cryostat sections.

08 November 2016 734 3 View

How to culture the giardia?

How to culture the giardia. I know I need to use the TYI-S-33 medium. But it is too expensive in the atcc company. Where can I buy the diamond's vitamin solution? It's there any tips to culture...

25 October 2016 4,020 3 View

How to genotype E6.5 mouse embryos?

I am collecting embryos for histological analysis and need to genotype them beforehand. In the past I have worked with older mouse embryos (E8.5 and up), where it is easy to remove the yolk sac...

21 July 2016 8,584 3 View

How-to on running factorial manova?

21 April 2016 8,391 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View