I know that for murine M2b SPHK1, LIGHT and Arginase1 heve been described as markers, but I cannot find other markers for human M2b except the IL-10 high and Il-12 low phenotype.
CCL1 has also been associated with M2b, and note that IL10 has been measured at both the protein and RNA level.
An interesting paper from last year suggested that the expression of several "passenger strand" miRNAs was significantly altered in M2 subtypes. See https://www.researchgate.net/publication/224878965
It would be good to see confirmation of this work, especially since the passenger strand miRNAs are comparatively scarce.
Article Identifying Functional MicroRNAs in Macrophages with Polariz...
Stella, i really dont know if it is feasible or not. But this paper shows that iron handling proteins are differently expressed during macrophage polarization. Maybe, mAb directed against these proteins could ease the identification of M2. If you give it a try, please let me know as i am interested in the same.
"Polarization dictates iron handling by inflammatory and alternatively activated
macrophages"
Hi Tomas,
I am interested in the so-called M2b macrophages and I am afraid the paper you mention refers to the M2a (differentiated by IL-4). Moreover this study is with mouse cells which are well characterized by other markers, whereas it seems that still robust, reproducible surface markers for human M2b macrophages have not, yet, been identified.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology