My project is to develop an in vitro cell culture model by dissociating a fresh salivary gland biopsy, culture the cells and perform viability assays using flow cytometry every 24 hours.
My question is whether it is possible to store the fresh intact biopsy for about 12 hours before I start the dissociation procedure. Do you think that will have a negative effect on the cell viability afterwards?
Thank you!
Emma
Sure, if you perform this method in any case via the same procedure (12 h at 4°C) the results you get should be comparable but may differ from other data published in different papers /(using different protocolls, procedures).
Andreas Eisenreich thanks for your answer. I guess I can give it a try and compare the results to those I get after I perform the biopsy dissociation immediately.
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