I am trying to express a membrane protein that, in the literature, was fused to a TrpLE tag. I am having trouble finding the actual sequence or a plasmid that encodes the TrpLE leader sequence. I would appreciate any information on how to track down the sequence or a plasmid that encodes the sequence.
Thanks!
Chad
You can find the TrpLE sequence just from the E. coli sequence of the Trp operon, but that really doesn't tell you exactly what is in your plasmid. If you don't know the origin of your plasmid then the fastest thing you can do is just sequence that region, you will have your answer in 2-3 days.
Hi Michael,
Thanks for the info. I am attempting to construct an expression plasmid with the appropriate sequence. I have looked in AddGene and other commercial sources with not much luck.
Best,
Chad
Did you find your answer? You may find the following publication useful.
Article Targeted expression, purification, and cleavage of fusion pr...
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