Does anyone know what is the best protocol for digest fragment of mice kidneys?

Alfredo Procino @Alfredo_Procino2

11 November 2013 1 9K Report

I must digest a fragment of mice kidney and I'd like to know what's the best collagenase.

Craig Lum

I gather your interest is in isolating DNA or RNA for study?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Alfredo Procino's questions See All

Does anybody know how to extract total RNA, mRNA, and lncRNA from human serum, plasma?

I have to extract miRNA from human serum.

10 November 2013 6,937 2 View

Can anyone suggest me a correct protocol to isolate adipocytes and preadipocytes from fragment of human white adipose tissue?

I have to study the role of wnt gene in human adipocytes.

10 November 2013 3,356 1 View

Can anyone suggest to me a correct protocol to isolate adipocytes and preadipocytes from fragment of human white adipose tissue?

I have to study the role of WNT genes in human adipocytes.

10 November 2013 6,960 2 View

Does anyone what the best kit is for RNA extraction from small fragments of tissue?

I'd like to know if there are kits for RNA extraction from small fragments (human biopsy) of adipose, bone, liver, prostate tissue etc..

01 February 2013 7,441 0 View

Does anyone experience with Qubit 2.0, this instrument is right for RNA quantittion?

Life Tech

10 November 2012 2,447 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

In the situation below, can I apply braun and Clark thematic analysis?

I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).

04 August 2024 8,798 7 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View