Anyone having experience with AKTA pure system of protein purification?

I have some questions regarding the AKTA pure system, mainly about the chromatogram.

03 April 2019 2,721 4 View

Is there someone willing to help me with my Bioinformatic course?

I need explanation on some specific concepts, in order to get a general understanding mainly in structural bioinformatics. And a suggestions on guides to use integrative software.

11 December 2018 9,868 10 View

Protocol for extraction of proteins from liver and pancreas of the mouse?

It doesn't matter if different protocols should be performed for the two organs. All the proteins are in the interest, as a quantitative proteomics experiment will be performed on them.

10 November 2018 7,020 3 View

Gibson cloning : Everything about it, principle in detail, practical aspect, advantage, disadvantage compared to other cloning methods.

Dear colleges! I am currently working with Gibson cloning, however I am pretty new on this. I would like some recommendations : articles, publications, your personal experience which describes in...

01 January 1970 8,376 4 View

Drug discovered but not developed?

I am interested to have some cases/examples where scientists come out with an efficient drug against a disease marker (successful clinical trials), although at the end they realized that the cost...

01 January 1970 5,146 6 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Why does Reaction Wood acts opposite in gymnosperm and angiosperms?

I am wondering why the Gymnosperms have an opposite behaviour that Angiosperms in the Reaction Wood (RW). Are there some new findings regarding that? Studies say that causal agents can be the...

10 January 2021 8,744 4 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View