Recently, I'm constructing the colon cancer mouse model by using Tyler Jacks lab's Apcfle1-15 mice which we get from the Jackson Lab. We need mating the homozygous Apcfle1-15 with our knockout mice. But we notice that the Jackson Lab's genotyping protocol cannot distinguish heterozygous from homozygous animals. And when I look up Tyler Jacks's oncogene paper(Complete deletion of Apc results in severe polyposis in mice), I didn't find anything to help with the genotyping primers design.
So, I'm looking forward to some information useful to genotype the homozygous Apcfle1-15 .
Thank you very much.
http://jaxmice.jax.org/protocolsdb/f?p=116:2:::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:15060,009045
http://www.nature.com/onc/journal/v29/n12/full/onc2009457a.html
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