Might help!

1. Germ cells of the mouse testis express P450 aromatase.

H Nitta et al, 1993 - press.endocrine.org .. Fractions containing pure populations of germ cells were pooled, pelleted, and frozen until used for protein extraction or RNA isolation. ... Microsomal fractions of germ and interstitial cells were

used because this procedure reduced ... A, Total cellular protein was extracted from .

2. Isolation and characterization of rat testis H1t. An H1 histone variant associated with spermatogenesis.

SM Seyedin et al, Journal of Biological Chemistry, 1980 - ASBMB

... 2, track e). When the HMG-depleted chro- matin was then extracted with 0.6 M NaCI, both Hlt ...D, 0.2 M HzSO, extract; E, 0.3 M NaCl extract; F. 0.6 M NaCl extract of 0.3 ... Extraction of testes that had remained in the abdomen for 2 weeks showed the complete disappearance of Hlt

This is the original article describing the isolation of different germ cell types from mouse testis using the STA-PUT procedure:

Dev Biol. 1976 Mar;49(1):119-31.

Separation of mouse spermatogenic cells by sedimentation velocity. A morphological characterization.

Romrell LJ, Bellvé AR, Fawcett DW.

PMID: 176074

Thank you

https://www.jove.com/video/53389/

Here is a video perform the isolation Procedure.