We want to deliver human cord blood mononuclear cells through artery. As we are concerning about this will cause microvascular thrombosis, so we would like to know the diameter range of these cells. But it seems like hard to find the information. Thanks.
Perhaps the pragmatic approach, especially given your specific concern, is simply to run CBC on some of your samples? I would recommend one of the higher-end clinical instruments that offers many reporting parameters. Alternative would be to get someone to run flow cytometry with one of the mononuclear cell labels, and distribution of cell diameter should be a relatively straightforward parameter.
I guess CB-MNC will not differ in size from PB-MNC so dramatically. So this woul imply the risk of thrombosis is not given. Even if you plan to inject in mice there are numerous publications about transplantation of MNCs without any problem.
So I would think size is comparable with other sources of MNCs
Regards
Sven
Primary mononuclear cells (MNCs) derived from the umbilical cord blood (CB) include hematopoietic lineage cells such as lymphocytes, monocytes, stem and progenitor cells as well as mesenchymal stromal cells. MNCs are isolated from CB using density gradient separation. The cord blood mononuclear cell are not differ in size from peripheral blood mononuclear cell.
First, UCB MNC contain lymphocytes, monocytes and other cells fractions, as in adults. The blood is enriched for cells with stem cell like properties, however, these cells do not differ in size.
Second, based on the info above, you shall not fear from thrombosis - these cells are made to make it through capillaries. This could be the cause for larger cells, like mesenchymal cells.
Third, you can induce microthrombosis by other manipulation: if you damage the internal surface of the artery, in you do not have proper anticoagulation during vessel surgery, if your cells make clumps...
See, there are many technical issues.
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