Refer the following link and attached article

BIOCOMPATIBILITY EVALUATION OF NICKELTITANIUM SHAPE MEMORY METAL ALLOY

http://herkules.oulu.fi/isbn9514252217/isbn9514252217.pdf

Most probably you can do cytotoxicity test using MTT or MTS assay on the metal samples to know the preliminary biocompatibility of the materials. I have used PrestoBlue last time. 

What is MTT or MTS.........? can you suggest me where i do this test.....any laboratory or any institutes.......??

Maybe you can refer here for the procedures. https://www.researchgate.net/publication/270884069_The_Effect_of_Micromachining_Process_to_the_Biocompatibility_of_3C-SiC_Membranes?ev=prf_pub

I am not sure about the nearest facilities to your place. Wish you the best Amit.

Conference Paper The Effect of Micromachining Process to the Biocompatibility...

Nasir Sir...

It is humble request to you If i courier you my sample then you check the biocompatibilty for the same..??

Dear Amit,

For evaluating the biocompatability in vitro, there are two kinds of tests you can apply

1-Direct contact assay; means to culture cells on the surface of your materials and this cells should be the same cells that will interact with your material in vivo. Simply the choice of which kind of cells you can use depends on the application of your material such as osteoblast cells in case of bone application, endothelial cells in case of vascular application, cardiomyocytes in case of cardiac applications.

In case of direct contact assay, you can evaluate

-The cell attachment on the material surface after different time points using scan electron microscope.

-The cell proliferation using MTT assay or DNA quantification

-You can also evaluate the function by immunofluroscence staining

 2- Indirect contact assay; In that test you will immerse your materials in media at a final concentration of 5 mg {your material}/ml {the media that you gonna use according to the cell type} for 12-72hrs to check whether your material can leash or release some toxic elements into media or not. You can call this media as a preconditioned media.

First culture the cells using normal media. Allow the cells to attach (needs approximately 12-24hrs), change the media and use your preconditioned media. You can check the response of cells to that preconditioned media using MTT assay or any cell viability assay. Afterwards you can try to compare your result with negative control group (cells cultured with normal media) and positive control group (cells cultured in presence of toxic material such as 20% dimethyl sulfoxide). Please check the attached paper!

Best Regards,

Kamal

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