I'm working on transmission of endophytic fungi in grasses. I need to observe the fungal hyphae growth in grass tissues. Hence, I need an best protocol for the study.
A Rapid Staining Method for Detection of Endophytic Fungi in Turf and Forage Grasses
January 1988Phytopathology 78(2)
DOI10.1094/Phyto-78-237
D. C. Saha
Hello Nischitha
Use a clearance solution ((0.15% trichloroacetic acid [w ⁄ v] in ethanol: chloroform [4:1; v ⁄ v]) for 48 h, with the solution being changed once during this time. Then wash 2 *15 min with 50% ethanol, 2 * 15 min with 50 mM NaOH and 3 * 10 min with MilliQ H2O, subsequently followed by 30 min of incubation in 0.1 M Tris⁄ HCl (pH 8.5). Samples were then either stained immediately or stored in 50% (v ⁄ v) glycerol se mentioned in the following references. Good luck
Dear Nischitha, i agree with Jawad K. Abood Aljanabi ,agood answer. With best regards
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