Dear colleagues,
I am trying to find an antibody to cytochrome c oxidase subunit 4 that crossreacts with oysters (Crassostrea gigas). Does anyone have an antibody they can share or suggestions to try?
I found this antibody in AbCam that crossreacts with vertebrates and Drosophila - perhaps someone has it and can share with us a few microliters to try out?
Anti-COX IV antibody [20E8C12] (ab14744)
Thanks!
Inna
Hi Inna,
Invitrogen Antibodies (Thermo Fisher) has a mAb (1D6E1A8) that they indicate is cross-reactive with mollusks. Here are our listings for anti-COX4 Abs:
https://www.linscottsdirectory.com/antibodies?q=COX4
follow "more info" to supplier's datasheet.
Hope this helps.
Hi
in silico check of the sequence ( BLAST) may help as a preliminary step or could corroborate the interpretation of stain. Of course a mAb is more specific than a polyclonal.
Good luck
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