How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to analyse data without control group in meta-analysis?

I am interested in knowing the treatment effect in my study. I have data (mean, SD) for pre and post treatment.

01 March 2020 3,572 2 View

The pH differential method - how do i calculate the concentration of anthocyanin in a dried extract?

27 February 2020 9,573 5 View

How to begin slicing sections after a period in cryoperservation?

I have preserved large sections of sheep brain tissue in an ethylene glycol solution for later use. I'm hoping to take the sections out of solution and then slice on a microtome or cyrostat. How...

29 January 2020 1,884 3 View

Please i need an expert help on how to convert amino acids results expressed mg/l to residues??

Please what formula should I use to convert amino acid compsition results expressed in mg/l to residues? I ran a HPLC amino acid profile of a protein sample, the results came in mg/l but I need to...

18 January 2020 5,765 1 View

Is anyone aware of a study that compares the discourse around intra-European mobility and integration for Eastern and Western Europeans?

I am looking for a study that compares the discourse around intra-European mobility and integration for Eastern and Western Europeans. Mobility of Western Europeans is not addressed in public...

20 August 2019 3,036 2 View

How does one calculate the % metal extraction, distribution ratio for each pH value, log D and pH1/2 from log D vs. pH plot from AAS results?

21 May 2019 8,115 3 View

In how many days maximum should I change the medium (L15) of breast cancer cells ?

01 February 2019 9,569 2 View

Have you used Covidence for systematic reviews before?

I came across Covidence for systematic reviews, which is the tool of choice for Cochrane, and was wondering if actual users can share their experience with the tool. It looks like a pretty...

06 August 2018 8,846 11 View

What would cause squared multiple correlation to be greater than 1?

I am testing a SEM model and one of my endogenous variable is defined by two observed variables (GPA and ability). The problem is that the squared multiple correlation being returned on GPA is...

21 June 2018 5,335 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View