I am looking for an antibody to demonstrate changes in the neuronal dendrites in the penumbra of an ischemic lesion of the visual cortex of the marmoset monkey. Tissues will be fixed in 4% PFA and cryosectioned (40um).
Synaptophysin or neuronal filament antibodies might also be useful markers. If you have expertise, I consider Golgi staining as one of the best methods to understand the density of neuronal dendrites. However, it is not immunostaining and the method is quite complex and requires a level of expertise. All the best.
Two other choices may be vimentin staining or alpha-tubulin. In the context of regenerating axons, vimentin staining is very strong, but I do not know if it extends into the dendrites. We use alpha-tubuin staining in peripheral muscle to detect new nerves, but in the brain there may be too many. Good luck.
You can try MAP2 antibody. It labels all parts of the neuronal densrties and also cell bodies. You need to use tissues from normal or sham animals as a control.
If it can help, the Neuron specific beta III tubulin (rabbit) antibody from abcam is working very well to analyse the organisation of neuronal dendrites. I am using it in a mouse model of focal ischemia...
Map2 is a bad antibody for visualising dendrites after injury as it disappears from distal dendrites following neurotrauma
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