Following ischemic injury to the visual cortex, we have observed a cohort of neurons that have nuclear vacuolation. These cells do not appear pyknotic and nuclei otherwise normal.
I am looking for an antibody to demonstrate changes in the neuronal dendrites in the penumbra of an ischemic lesion of the visual cortex of the marmoset monkey. Tissues will be fixed in 4% PFA...
11 December 2012 2,826 8 View
Once a PhD and success in research meant a great career ahead but now the tides appear to be changing and advice from even the funding bodies is that researchers should challenge themselves and be...
08 September 2012 4,020 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...
17 July 2024 1,225 0 View
The main differences
14 July 2024 3,158 4 View
cerebral palsy rehabilitation
10 July 2024 5,220 2 View
I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...
30 June 2024 1,234 2 View
I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...
23 June 2024 4,083 1 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... How does prolonged ischemia lead to myocardial necrosis?
15 June 2024 6,589 1 View