Does anyone know of a way to crosslink polymers containing sulfonate groups?

More Grzegorz Celichowski's questions See All

Do you really know the second law of thermodynamics? Can you formulate it precisely? Do you think this is a fundamental principle?

All thermodynamics textbooks say that the Clausius formulation and the Kelvin formulation are equivalent to the entropic formulation - and this is proven. Meanwhile, there is only one proof...

16 November 2023 8,453 58 View

Which tweezers for piranha solution?

Hello, I am planning to work with acidic piranha solution made from sulphuric acid 98% and hydrogen peroxide 30% (volumetric ratio 3:7). I worked with this solution before, but this time I want...

24 May 2023 8,273 1 View

Ti6Al4V and Ti6Al7Nb PEO coatings don't conduct?

Hi, I recently did the plasma electrolytic oxidation coatings on Ti6Al4V and Ti6Al7Nb. When it comes to potentiodynamic tests - they're getting stuck because of the curve is below 0 current...

19 May 2023 1,165 4 View

Southern blotting - why is low-stringency wash used?

I have a question regarding the hybridisation step in Southern blotting. I understand that once the probes are added, the membrane is washed in buffer of increasing stringency to increase the...

17 November 2022 961 1 View

How to increase glycolysis/glycolytic capacity in vitro while using Seahorse XF?

Dear all I am performing the mitostress seahorse assay in aortic endothelial cells. I am looking for a good positive control that would increase the glycolysis/glycolytic capacity when I treat the...

11 October 2022 4,349 1 View

What are best positive and negative controls for glucose uptake experiment?

Hello, I'm trying to determine the amount of glucose consumption using 14C-labelled 2-DG. What would be the good controls to use in the experiment? For negative I planned to use low glucose...

23 August 2022 5,211 2 View

The refractive index of 8YSZ, La2Zr2O7 etc.?

Where I can find the refractive index of 8YSZ, La2Zr2O7, Gd2Zr2O, Sm2Zr2O7, Nd2Zr2O7 or other zirconates of rare earth elements?

11 August 2022 8,315 4 View

Does anyone have data how many total protein can I isolate from different tissues with Ripa buffer??

As above. Especially I'm iterested in fat, muscle, liver and PBMC cells.

27 February 2022 9,736 1 View

GC-MS analysis of essential oils composition - types of columns?

Is it possible to replace the Agilent HP-5MS 30m x 0.25mm x 0.25μm column with the TraceGOLD TG-5 SILMS 30m x 0.25mm x 0.25μm column in GC / MS analysis of essential oils?

31 January 2022 1,187 6 View

Are there any other solvents for PETN (pentaerythritol tetranitrate)...?

Which at the same time are much less miscible with water (than acetone or DMF)... Will be grateful for some quantitative data/references on that.

29 September 2021 3,611 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View