Could anyone help me with a SNP primer desing?

16 February 2021 3,049 3 View

Can you share your online teaching-learning experience during this pandemic period?

I have started online classes for undergrad, master, and Ph.D. students last January 16 up to the end of May. I have request students to allow recording classes and to discuss the process at the...

14 September 2020 4,466 23 View

Does someone have a protocol for glucose uptake experiments in vivo?

I am planning to run a glucose uptake experiment in mice (In vivo). I would like to inject H3-2DG and just measure the percent of H3 in different tissues. No clamp experiment, but I was thinkMing...

18 February 2020 9,531 3 View

Can I use different rotation tecniques to compare extract factors in different years?

Dear researchers, I obtained a rotation matrix (varimax) in my factor analysis with correlation no higher than 0.20 for my 2010 data. However, for my 2014 data, with varimax rotation, I obtained...

20 January 2020 5,393 2 View

Isolating high molecular weight DNA from a single feather?

I'm attempting to extract DNA from a single retrix from a small sparrow. I have upwards of 200 samples but only one feather per individual. The feathers have no visible blood spots on them. I've...

14 August 2018 5,190 2 View

Can I store genomic DNA at -20 C for a year without degradation that could affect qPCR runs by that time?

Hi, I will need to run qPCR on genomic DNA samples in about a year from now. Right now isolated DNA is preserved at -20 C. Does anyone know if I should do something else with the samples so that...

30 November 2016 8,366 6 View

Does anybody know if the Sysmex XT-2000i analyser can be connected to LIMS system?

14 January 2015 1,416 2 View

Is there any specific RNAse for short dsRNA?

18 November 2014 1,038 1 View

How to check siRNA molecule thermodynamics?

I've been questioned about the thermodynamics of my siRNAs molecules. It is well described that the strand with less stability at 5' has a higher probability to be incorporated at RISC complex....

18 May 2014 5,472 1 View

Collaborative learning - Aprendizagem colaborativa

How can technology-mediated education strengthen collaborative learning in public schools at low cost to governments? Como o ensino mediado por tecnologia pode fortalecer a aprendizagem...

01 January 1970 8,449 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View