We have a Lancer vibratome 1000 in our lab that we are trying get up and running. It is my understanding that these machines can last a while and still perform well. Our issue is that despite trying numerous control settings and different types of tissue, when we reduce sectioning thickness to 50 um, the machine only takes tissue slices every other pass, and the sections we do get seem to be of non-uniform thickness.

Similar questions and discussions