I am trying to differentiate motor from sensory nerve fascicles in peripheral nerves. Does anyone know if there is a specific stain?
yes there is, look for Choline Acetyltransferase. It is however a quite tricky stain.
https://en.wikipedia.org/wiki/Choline_acetyltransferase
Thank you for your reply. However, I want to defferentiate motor from sensory nerve fascicles in cadaver specimens. Will it work?
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...
25 December 2020 6,258 4 View
You are invited to participate as expert panel member in our consensus study on International Online Collaboration Competencies (IOCC). The purpose of this study is to build consensus on the key...
11 November 2020 9,715 1 View
I'm taking some measurements using Raman spectroscopy of healthy pig eye samples using a new excitation wavelength and my supervisor said to choose a sample size to allow for spec/sens of 85%....
01 November 2020 8,611 6 View
Hello, I am running an ANOVA between 3 groups (Group 1 N=148, Group 2 N=147, Group 3- N=144; equal variances assumption met) . I am having difficulties in deciding upon the most adequate post-hoc...
21 October 2020 2,182 5 View
I was able to find some applications of deep watershed for image instance segmentation, but nothing for rocks. Could you please recommend any study?
07 September 2020 5,731 8 View
I am using chlorosulfonic acid in chloroform (inert, 2 hr, RT) to para-sulfonate benzyl groups on a dibenzyl ether. The work up is extraction with water. The reaction, however, does not seem to...
04 August 2020 4,446 7 View
Investigating the difference in business strategies between ethical marketplaces and traditional online marketplaces such as amazon
27 July 2020 7,302 3 View
Dear users of nanoindenters, I was wondering what procedures do you usually use to clean your diamond nanoindentation tips? I know that there is nothing specifically described but some internal...
08 July 2020 5,926 5 View
At the moment our laboratory would like to buy portable NMR spectrometer and we would like to see some real not promo spectrograms. Could those who constantly work with such devices share spectra...
22 June 2020 8,144 6 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...
03 March 2021 961 3 View
I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.
02 March 2021 1,516 4 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View